RESEARCH OF THE ACTIVITY OF RRNA GENES IN NUCLEAR ORGANIZERS OF BLOOD LYMPHOCYTES KINGS OF UKRAINIAN BREEDING
Abstract
The study of animal nucleolar organizers makes it possible to assess the level of functional activity of 18S/28S ribosomal genes involved in protein biosynthesis. The aim of the work was to study the signs of nucleoli activity in the interphase blood cells of rabbits of different breeds of Ukrainian breeding.
In the experiment, 4-month-old female rabbits of the Poltava Silver breed (n = 30), Californian (n = 25), and their hybrids (n = 21) were used. Zones of nucleoli in intact blood lymphocytes were studied according to the method of Howell and Black (1980). The preparations were stained with a solution of 50% AgNO3 with the addition of a 1% solution of formic acid and incubated in a humid chamber at a temperature of +60 °C. Microscopy was performed using a microscope "ZEISS, Germany" (magnification 10×100). At least 200 interphase cells were studied in each animal. The activity of nucleoli was evaluated according to the parameters: the average number of nucleoli in the nucleus (nNR), the total area of the nucleus in the nucleus (ΣSNR, μm2), and the share of the area of the nucleolus in the area of the lymphocyte nucleus (hΣSNR, %). Statistical analysis was carried out using standard variation statistics programs included in the "STATISTICA" (2020) program package. The average number of nucleolar cells varied from -1.70±0.08 in California rabbits to 5.90±0.29 in hybrid animals. A statistically significant difference (p<0.05) was found between the experimental groups of purebred and hybrid rabbits. The coefficient of variation of the average number of nucleoli per cell was at the average level of variability: 20.58% in rabbits of the Poltava silver breed, 19.50% in the California breed, and 16.49% in hybrids. The total area of the nucleus in the cell in all experimental animals varied from 5 μm2 in one of the individuals of the California breed to 12 μm2 in individuals of hybrid origin. The share of the area of the nucleus from the area of the nucleus in Poltava silver, California, and hybrid rabbits was 26.10±1.80%, 24.30±1.62, and 29.40±2.50, respectively.
Correlation analysis revealed a statistically significant relationship between the number of nucleoli per cell and the total area of the nucleolus in the cell nucleus (r = 0.54, p<0.01) and between the number of nucleoli per cell and the fraction of the area of the nucleolus from the area of the nucleus (r = 0.28, p<0.05) in Poltava silver rabbits.
Polymorphism was established according to the studied parameters of nucleoli activity in intact peripheral blood lymphocytes of Poltava silver, California, and hybrid rabbits.
The existence of a statistically significant difference between the research groups of purebred and hybrid rabbits in terms of the number of nuclei per cell, the total area of nuclei per cell, and the fraction of the nucleus from the area of the lymphocyte nucleus has been proven.
The results of a comparative analysis of the studied parameters of the activity of nucleoli in peripheral blood lymphocytes of rabbits of the Poltava silver, California, and hybrid breeds indicate a higher activity of nucleoli in animals of hybrid origin.
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