THE EFFECT OF NANOPARTICLES OF MICROELEMENTS ON THE HEMATOLOGICAL INDICATORS OF RABBITS DURING THE DEVELOPMENT OF HEAT STRESS

Abstract

The use of traditional technological methods to reduce heat stress in rabbit breeding is ineffective, unlike various dietary supplements in the diet. Experiments

were carried out on young rabbits of the Thermon White breed from 35 to 78 days of age. The rabbits were kept in a vivarium, where conditions of elevated ambient

temperature (moderate and severe heat stress) were created, with a temperature- humidity index ranging from 28.1 to 29.9 °C and relative humidity from 84.3 to

85.7%. For the study, a control group of rabbits was formed, which were kept on standard granulated feed produced on the farm and given water ad libitum. Rabbits

in groups I, II, and III consumed the same feed as the control group, but for 24 hours they received the following with their water: Group I – zinc citrate – 60 mg/l or 12

mg/kg of body weight; Group II – selenium citrate – 300 μg/l or 60 μg/kg of body weight; Group III – germanium citrate – 62.5 μg/l or 12.5 μg/kg of body weight. The

blood parameters of rabbits were examined on the 14th day of the preparatory period and on the 14th and 29th days of the study under conditions of moderate and

severe heat stress. It was found that the most pronounced effect on changes in the hematological parameters of rabbits' blood was observed when zinc citrate and

selenium citrate were added to the diet. These changes were more pronounced under conditions of severe heat stress and were characterized by higher levels of erythrocytes, hemoglobin, hematocrit, leukocytes, and their individual forms throughout the study. It was found that the most pronounced effect on changes in hematological parameters of rabbits' blood was observed when zinc citrate and selenium citrate were added to the diet. These changes were more pronounced under

conditions of severe heat stress and were characterized by higher levels of erythrocytes, hemoglobin, hematocrit, leukocytes, and their individual forms throughout the study. The effect of germanium citrate was less pronounced compared to other supplements used in the control group, which was reflected in increased hemoglobin levels under conditions of moderate heat stress and the number of leukocytes, monocytes, and granulocytes under conditions of severe heat stress on the 14th and 29th days of the experiment.